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The lipid in the fish roe likely can have an impact on the accessibility of proteases to proteins. Fish roe having a substantial lipid content could be fewer competently hydrolyzed due to the hindering influence of the Excess fat molecules, which Restrict the protease usage of the protein. Fatty acids may bind to proteins and alter their three-dimensional construction, which influences the proteases’ conversation with proteins [37,38]. One more reason that could demonstrate the observed variations in the hydrolysis potential of the three proteases is their hydrolytic specificity. The protease supply as well as substrate used for the hydrolysis procedure can effect the rate along with the extent of your hydrolysis process. By way of example, Kumar et al. [39] attained trypsin from 3 diverse fish Catla catla, Labeo rohit, Hypophthalmichthys molitrix were being accustomed to hydrolysis casein, soybean food, silver carp, and Chilean fish food and The end result demonstrated which the DH is affected through the enzyme supply and substrate composition [39].
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